I upgraded the stem cell section (8) longevity3.html to include more information on reprogramming somatic cells to become pluripotent stem cells, naming the 4 genes insterted into the genome to enable this in mouse skin cells. Then the cloud cover seemed to respond by showing a WUF (Wichita State University, University of South Florida, Friedrich Wilhelms Universitat) with a heart of gold uttering things above Wichita, Kansas. This is a typical I see you, do you see me? signal from the spirit of the cloud cover that seem to haunt us with respect to news releases. What I added to the file to stimulate this response was:

"Note that it has recently been possible to prepare embryonic and other stem cells from skin cells, so that all stem cells you use can be perfectly DNA-matched and legal. The results were seperately published by Dr. Shinya Yamanaka [Links, Images] of Kyoto University and UCSF in Cell [Papers, Links, images] and James Thomson of the University of Wisconsin in Science [Papers, Links, images]. Dr. Yamanaka showed that pluripotent stem cells can be generated [Books, Papers] from mouse embryonic fibroblasts and adult mouse tail tip fibroblasts by the retrovirus-mediated transfection of four transcription factors [Books], Oct3/4, Sox2, c-Myc, and Klf4. "

Well, there you are, just as I have told you for years. Behind every Wolfish editor lurks a Pirana Fish complex. This is probably a message from On High explaining that I look more a genius and less like a predator when my work is altogether original.

A study note may have unusual psychic impact:

January 26, 2009
hTERT promoter [Links, Books, Patents/hTERT promoter, Papers, Amazon, Books/human gene promoter and repressor design, Books/transcription promoters]. See Morin, et.al, the Aug. 17, 2004 Geron patent Telomerase promoter driving expression of therapeutic gene sequences. See also M Wick, D Zubov, G Hagen, 1999, Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT), Gene, Volume 232, Issue 1, 17 May 1999, Pages 97-106. Furthermore, don't miss Cong, YS, The Human Telomerase Catalytic Subunit hTERT: organization of the gene and characterization of the promoter, Human Molecular Genetics, 1999, vol.8, no.1, 137-142. See also Yu-Sheng Cong, Woodring E. Wright, and Jerry W. Shay, Human Telomerase and Its Regulation and Izumi Horikawa, P. LouAnn Cable, Cynthia Afshari and J. Carl Barrett, 1999: Cloning and Characterization of the Promoter Region of Human Telomerase Reverse Transcriptase Gene , Cancer Research, 59, 826-830, February 1, 1999 [Papers, Books]. See also Telomerase, Aging and Disease (Advances in Cell Aging and Gerontology) by M.P. Mattson, Elsevier, 2001. Note c-Myc activates hTERT promoter [Links, Books, Papers]. Dr. Michael Fossel suggesting using c-Myc plasmids (perhaps with supplemental controls, such as telomerase inhibitors, of course) to activate hTERT in human cells for life extension in Cells, Aging, and Human Disease. "The hTERT promoter lacks TATA and CAAT boxes and is in a CpG island with an E-box (CACGTG) binding site and sites for Sp1 and several for c-Myc...hTERT and c-Myc are expressed in actively dividing cells, but down-regulated in non-dividing cells. ... C-Myc rapidly and directly induces hTERT expression." (M.Fossel, p.286). See also Theodora R. Devereux, Izumi Horikawa, Colleen H. Anna, Lois A. Annab, Cynthia A. Afshari and J. Carl Barrett, 1999. DNA Methylation Analysis of the Promoter Region of the Human Telomerase Reverse Transcriptase (hTERT) Gene, Cancer Research 59, 6087-6090, December 1, 1999. See also Joseph C. Poole, Lucy G. Andrews and Trygve O. Tollefsbol, Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT), Gene, Volume 269, Issues 1-2, 16 May 2001, Pages 1-12. Note that the hTERT promoter for the catalytic component of telomerase contains binding sites for the transcription factor Sp1 [Links, Papers, Books] and for the transcription factor c-Myc [Links, Papers, Books; Wikipedia/C-myc]. Binding sites for the estrogen receptor [Links] and the progesterone receptor [Links] are also found in the hTERT promoter, and it has also been reported that protein kinase C [Links, Books, Books/protein kinase C and telomerase] is involved in the regulation of telomerase activity. It has also been shown that Akt Protein Kinase [Links; Links/Akt Protein Kinase and telomerase, Books] is associated with two receptor sites on the hTERT promoter. See Sang Sun Kang, Taegun Kwon, Do Yoon Kwon, and Su Il Do, Akt Protein Kinase Enhances Human Telomerase Activity through Phosphorylation of Telomerase Reverse Transcriptase Subunit, J Biol Chem, Vol. 274, Issue 19, 13085-13090, May 7, 1999.

Fixing up this hTERT promoter section seemed to have psychic impact: I woke up on the morning after its preparation with Deck the Halls joyously ringing in my ears as like a distant response from pleased readers, as if from a chapter scene in A Christmas Carol by Charles Dickens.

The Ghost of Christmas Present. | DVD

Here it seems that the decision is not to pursue the creation of pups via the dog-style chase, but to fetch toys designed for Southern Comfort instead. The "M60" declaration "Hang the pups, pass me the cock" might have been a fine subtitle for this scene. It might picture reaching for the eye-opening DVD She Male Cock Party, which investigates alternatives to procreation filmed South of the Border, for instance. On the other hand, I wrote a letter to my brother Rick in Arizona to pick up a response of some kind, since he has not sent word for a while. Are he and his bride still alive and kicking? Music: Time After Time by Cyndi Lauper.

Erotic Hots Study Guide

Here George Washington, the spirit of galaxy M100 and the man on the 100-cent piece, seems to advise a descendant of the original American Revolutionaries by communicating through an image in the clouds. Note that a sidelock of hair seems to extend down from Washington DC to the Mexican border, as if to refer to a remark concerning something Mexican.
Aside: Visionary Bull #4

Well, you cook up some literary bread, but sometimes those muffins just don't seem to rise just right. So you cut them off and drop them into an aside buffer in order to be able to extract a few valuable nuggets from them later. The cloud cover responds by showing an Arkansas devil with a cone nose munching on some bread fed to him from above, the cone nose pointed over Wichita, Kansas. We note that the tip of his nose is cut off at the Wichita end, mirroring the humor of the decision. Thus a visionary I see you, do you see me? signal resembling "Roger, I read you, over and out." comes back from the cloud cover that I am processing on the other end. Sons of the Divine Wind can look forward to witnessing such phenomena, which seem to track us when we are headline news of some type. Then it seems the cloud cover reads us and writes back in cloudy symbols with its typical ACKNOWLEDGE pulse framed like "I see you, do you see me?", careful to show an elevated sense of humor, too. Note that there is another little cloudy head in Ohio biting the nose off the figure feeding the bread to the Arkansas fellow. This seems to remind us of the adage advising not to "cut off your nose to spite your face." As we write, gradually turning the inside into the outside, the outside begins to signal back. When we discover exhibits and signs from our friends on Earth alluding to us, this is easily comprehensible. When it comes from "today's the brain" in the nebulosities of the cloud cover, however, we note that we are haunted by a rainy day ghost from a firmament of heaven driven by the "rays of the Sun".

The image seems to refer to Visionary Bull #4 and my recent practice of dropping half-baked literary pieces into a water background in narrow columns of brown on goldenrod. Note how the pot resembles the Feed Me Plant in Little Shop of Horrors, while the man with his head in profile against the pot resembles The Jolly Green Giant. Note how the Canadian lakes resembling an equals-sign and including Lake Winnipeg intersect his forehead. The equation lakes sign reminds me of my work in cosmology, theoretical physics, computational astrophysics, image processing, medical image processing, and electronic and computer engineering (Profile).