Fruitful Vine

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PREGNANCY TEST INSTRUCTIONS

TEST DESCRIPTION

One Step HCG Urine Pregnancy Tests are used for qualitative (visual) determination of hCG (Human Chorionic Gonadotropin) in urine specimens for early detection of pregnancy. HCG is a glycoprotein hormone secreted by developing placenta shortly after implantation. hCG can be detected in urine and serum of pregnant women as early as 6 to 15 days after conception. The early pregnancy tests are designed to screen very low hCG concentrations in this initial hCG development stage.

These tests are specifically designed for professional users (doctors, clinics, family planning centres) for determination of early pregnancy. Tests detect hCG in urine in a qualitative format with sensitivity of 20mIU/mL (International Standard Value). The test line is responsive in the range of 10-50 mIU producing a line directly proportional to concentration of hCG in urine. The specificity is 99.9% meaning that test is highly selective for beta-hCG glycoprotein. Tests have an expiry date of 2 years and can be stored in a dry environment ranging from 2 to 30 degrees Celsius. The products are manufactured in ISO 9001 facility and posses Health Canada and US FDA 510k clearance. The product performance has earned an international reputation for quality and reliability, and are distributed in over 70 countries worldwide.

FORMATS

The tests are available in 3 different formats:

1. STRIP

2. CASSETTE

3. MIDSTREAM

The format applications range from professional to domestic usage.



TEST PROCEDURE

STEP 1

Bring the test pouch to room temperature (18-30°C). Collect urine into a clean container (such as plastic cup). The best way to collect sample is by placing a cup in the middle of urination process. To begin testing, open the sealed pouch by tearing along the notch. Remove the test from the pouch when you are ready to use it.

STEP 2 (TEST FORMATS)

STRIP TEST

Immerse the strip into the urine with the arrow end pointing towards the urine. Do not immerse past the MAX (maximum) line. Take the strip out after 3 seconds and lay the strip flat on a clean, dry, non-absorbent surface (e.g., mouth of the urine container). Do not immerse for longer than 5 seconds.

CASSETTE TEST

Draw the sample into the provided pipette, and dispense 0.2ml (about 4 drops) into the round dropping well of the cassette.

MIDSTREAM TEST

Hold the round end of cover with one hand. Use the other hand to pull out the test device and expose the absorbent window. Point the absorbent tip downward and place the absorbent tip in urine stream for at least 10 seconds to be thoroughly wet. Otherwise, you can collect your urine into a clean cup and dip half of the absorbent pad into the urine for at least 10 seconds. Re-cap the device and place it on the clean, flat surface.

STEP 3

Wait for the colored bands to appear. Depending on the concentration of hCG in the test specimen, positive results may be observed in as short as 40 seconds. However, to confirm negative results, the complete reaction time (5 minutes) is required. Do not read the results after 10 minutes as this type of test is designed for rapid determination only.



INTERPRETATION OF RESULTS

Negative - Not Pregnant:
Only one color band appears in the control region. No visible band on the test region is present since hCG concentration is not present in urine or too low for detection.

Positive - Pregnant:
Distinct color bands appear on the control and test regions. The presence of both test and control lines indicate possible pregnancy. Color intensity of the test band may vary according to the concentration of hCG in urine. The higher the hCG concentration - the darker the intensity of the test line, hence higher the likelihood of pregnancy.

Invalid - Uncertain Results: No visible bands in the control and test regions. Make sure to follow the above specified instructions for optimum results.

The intensity of the test line is directly proportional to the concentration of hCG in the range of 10-50mIU. Test results may vary for different individuals depending on the concentration of hCG your body produces, as well as monitoring frequency, and testing technique. The best method is to test once a day, at a specific time, by closely following these test instructions.

PRECAUTIONS

1. For single in vitro diagnostic use only - use test only once for urine screening.

2. Do not use test kit beyond expiry date.

3. Keep away from moisture, direct sunlight, and children.

4. Fruitful Vine is not responsible for your test results. If you get inconsitent results or think you are pregnant, please see your doctor.

Note: This type of rapid test is used for preliminary qualitative screening of early pregnancy. It is usually used professionally to trigger other analytical procedures (blood test, ELISA, etc.) for quantitative determination of pregnancy conditions. If you suspect that you are pregnant, or have any concerns on pregnancy, it is recommended that you see your physician.

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